Biochemistry and Molecular Biology Laboratory Manual
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Description
Book Introduction
The field of life science is particularly experimental, and without experiments it is impossible to elucidate the mysteries of life phenomena.
Even if you do the same experiment, if you do it while understanding it, you are a scientist. If you do it without understanding it or do it without thinking, you are not a scientist but just an experiment assistant.
『Biochemistry and Molecular Biology Laboratory Manual』 is a book that contains as much advanced experimental content as possible at the undergraduate level in biochemistry and molecular biology, which are major experimental subjects in the life sciences, and is designed to be easily understandable.
The Introduction section at the beginning of each chapter provides a relatively detailed explanation so that students can first understand the content and then conduct experiments.
Each chapter is linked to a video and a Virtual Lab is implemented, so you can implement experiments while watching the video.
Additionally, questions and references at the end of each chapter allow for more in-depth study.
Even if you do the same experiment, if you do it while understanding it, you are a scientist. If you do it without understanding it or do it without thinking, you are not a scientist but just an experiment assistant.
『Biochemistry and Molecular Biology Laboratory Manual』 is a book that contains as much advanced experimental content as possible at the undergraduate level in biochemistry and molecular biology, which are major experimental subjects in the life sciences, and is designed to be easily understandable.
The Introduction section at the beginning of each chapter provides a relatively detailed explanation so that students can first understand the content and then conduct experiments.
Each chapter is linked to a video and a Virtual Lab is implemented, so you can implement experiments while watching the video.
Additionally, questions and references at the end of each chapter allow for more in-depth study.
- You can preview some of the book's contents.
Preview
index
Preface 0
PART 1 Biochemistry 1
CHAPTER 1 Preparation of Buffer Solutions and Amino Acid Titration 3
01 pH and buffer solution 4
CHAPTER 2 Qualitative and Quantitative Analysis of Amino Acids and Proteins 15
01 Methods for Protein Concentration Determination 16
02 Separation and qualitative analysis of amino acids 27
CHAPTER 3 Protein Overexpression and Chromatography 37
01 Cell and Protein Harvest 38
02 Protein Overexpression and Purification 41
03 Purification of Taq polymerase 51
04 Protein purification using MBP-Taq affinity chromatography 60
05 Size Exclusion Chromatography (Gel Filtration Chromatography) 64
CHAPTER 4 Enzyme Activity and Kinetics 73
01 Absorptiometry 74
02 Enzyme Kinetics 81
CHAPTER 5: Lipid Extraction and Qualitative Analysis 93
01 Lipid Extraction and Qualitative Analysis from Animal and Plant Tissues 94
CHAPTER 6 Carbohydrate Separation and Quantification 107
01 Isolation and qualitative analysis of carbohydrates from liver tissue 108
CHAPTER 7 SDS-PAGE and Western Blot 117
01 SDS-PAGE 118
02 Coomassie Blue Staining 127
03 Western Blotting Analysis 131
CHAPTER 8 Isolation and Analysis of Intracellular Protein Extracts 143
01 Cell Lysate Preparation 144
02 Reporter Gene Assay/ BCA Assay 151
CHAPTER 9 Peptide Finger Printing 157
01 Peptide Mass Fingerprinting I 158
02 Peptide Mass FingerPrinting II 169
PART 2 Molecular Biology 189
CHAPTER 10 Plasmid DNA Manipulation and Transformation 191
01 Restriction Enzyme/Agarose Gel Electrophoresis/Ligase 192
02 Restriction Mapping and Transformation of Circular Plasmid DNA 211
03 Plasmid DNA Preparation 221
04 Midi-Scale Preparation 229
05 Measuring the Transformation Efficiency of Competent Bacteria 241
CHAPTER 11 Reverse Transcription and PCR Reactions 245
01 PCR (Polymerase Chain Reaction) 246
02 Analysis of Gene Expression Changes Using Real-Time PCR Method 250
03 Mutagenesis using PCR 255
04 Reverse transcription-polymerase chain reaction analysis 262
CHAPTER 12 Transfection of Higher Animal and Plant Cells 273
01 Mammalian Cell Culture 274
02 Transfection on Mammalian Cells 279
03 Transfection of Animal Cells Using Calcium Chloride (CaCl2) 284
04 Transfection of Animal Cells Using Liposomes 289
05 Transformation of protoplast cells using PEG (Polyethylene Glycol) 295
CHAPTER 13 Isolation of Eukaryotic RNA and Genomic DNA 303
01 Isolation of Genomic DNA from Monolayer Cultured Cells 304
02 Isolation of Genomic DNA from Rat Tail Tissue 311
03 Plant Genomic DNA Isolation 319
04 Total RNA Isolation from Plants Using TRIzol 325
05 RNA isolation from monolayer cells using TRIzol reagent 332
CHAPTER 14 Cell Isolation and Analysis 339
01 Cytotoxicity Test (MTT Assay) 340
02 Isolation, staining, and flow cytometry of mouse spleen immune cells 344
03 Isolation of mouse blood immune cells using centrifugation 352
Search 365
PART 1 Biochemistry 1
CHAPTER 1 Preparation of Buffer Solutions and Amino Acid Titration 3
01 pH and buffer solution 4
CHAPTER 2 Qualitative and Quantitative Analysis of Amino Acids and Proteins 15
01 Methods for Protein Concentration Determination 16
02 Separation and qualitative analysis of amino acids 27
CHAPTER 3 Protein Overexpression and Chromatography 37
01 Cell and Protein Harvest 38
02 Protein Overexpression and Purification 41
03 Purification of Taq polymerase 51
04 Protein purification using MBP-Taq affinity chromatography 60
05 Size Exclusion Chromatography (Gel Filtration Chromatography) 64
CHAPTER 4 Enzyme Activity and Kinetics 73
01 Absorptiometry 74
02 Enzyme Kinetics 81
CHAPTER 5: Lipid Extraction and Qualitative Analysis 93
01 Lipid Extraction and Qualitative Analysis from Animal and Plant Tissues 94
CHAPTER 6 Carbohydrate Separation and Quantification 107
01 Isolation and qualitative analysis of carbohydrates from liver tissue 108
CHAPTER 7 SDS-PAGE and Western Blot 117
01 SDS-PAGE 118
02 Coomassie Blue Staining 127
03 Western Blotting Analysis 131
CHAPTER 8 Isolation and Analysis of Intracellular Protein Extracts 143
01 Cell Lysate Preparation 144
02 Reporter Gene Assay/ BCA Assay 151
CHAPTER 9 Peptide Finger Printing 157
01 Peptide Mass Fingerprinting I 158
02 Peptide Mass FingerPrinting II 169
PART 2 Molecular Biology 189
CHAPTER 10 Plasmid DNA Manipulation and Transformation 191
01 Restriction Enzyme/Agarose Gel Electrophoresis/Ligase 192
02 Restriction Mapping and Transformation of Circular Plasmid DNA 211
03 Plasmid DNA Preparation 221
04 Midi-Scale Preparation 229
05 Measuring the Transformation Efficiency of Competent Bacteria 241
CHAPTER 11 Reverse Transcription and PCR Reactions 245
01 PCR (Polymerase Chain Reaction) 246
02 Analysis of Gene Expression Changes Using Real-Time PCR Method 250
03 Mutagenesis using PCR 255
04 Reverse transcription-polymerase chain reaction analysis 262
CHAPTER 12 Transfection of Higher Animal and Plant Cells 273
01 Mammalian Cell Culture 274
02 Transfection on Mammalian Cells 279
03 Transfection of Animal Cells Using Calcium Chloride (CaCl2) 284
04 Transfection of Animal Cells Using Liposomes 289
05 Transformation of protoplast cells using PEG (Polyethylene Glycol) 295
CHAPTER 13 Isolation of Eukaryotic RNA and Genomic DNA 303
01 Isolation of Genomic DNA from Monolayer Cultured Cells 304
02 Isolation of Genomic DNA from Rat Tail Tissue 311
03 Plant Genomic DNA Isolation 319
04 Total RNA Isolation from Plants Using TRIzol 325
05 RNA isolation from monolayer cells using TRIzol reagent 332
CHAPTER 14 Cell Isolation and Analysis 339
01 Cytotoxicity Test (MTT Assay) 340
02 Isolation, staining, and flow cytometry of mouse spleen immune cells 344
03 Isolation of mouse blood immune cells using centrifugation 352
Search 365
Detailed image
GOODS SPECIFICS
- Date of issue: March 4, 2019
- Format: Springbook book binding method guide
- Page count, weight, size: 384 pages | 215*275*30mm
- ISBN13: 9791159431333
- ISBN10: 1159431337
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