Pharmaceutical biochemistry experiment
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Description
Book Introduction
It is designed to enable students to acquire practical and highly applicable experiments suited to their major by systematizing newly developed biochemical experimental techniques and currently conducted experimental tasks.
For each practical task, the principles of the experiment are described to ensure a clear understanding of the practical content, and examples are provided of how to convert the experimental results into data.
For each practical task, the principles of the experiment are described to ensure a clear understanding of the practical content, and examples are provided of how to convert the experimental results into data.
index
Ⅰ Protein analysis technology
Experiment I-1 Protein Electrophoresis (SDS-PAGE) and Western Blot
Experiment Ⅰ-2 Protein quantification using ultraviolet absorbance
Experiment I-3 Protein quantification by BCA (Bichinchominic acid) method
Experiment I-4 Protein quantification by Lowry method
Experiment I-5 Enzyme Kinetics
ⅡGene analysis technology
Experiment II-1 Polymerase Chain Reaction (PCR)
Experiment II-2 Transformation and Confirmation of Genetic Traits
Experiment II-3: Purification of Plasmid DNA
Experiment II-4 Restriction Enzyme Treatment and Plasmid DNA Confirmation
Experiment II-5 RNA extraction and quantification
Experiment Ⅱ-6 RT-PCR (reverse transcription polymerase chain reaction)
Ⅲ Clinical Application Technology
Experiment III-1 Sugar Search Reaction
Experiment III-2 Cholesterol Quantification - Modified Zak-Henly Method
Experiment III-3 Quantification of Alanine Aminotransferase (ALT) in Serum
Experiment III-4: Fractionation and protein quantification of experimental animals (mouse, rat)
Experiment III-5 Cell culture and viability measurement (Cell culture and MTT assay)
Ⅳ Reference
Experiment I-1 Protein Electrophoresis (SDS-PAGE) and Western Blot
Experiment Ⅰ-2 Protein quantification using ultraviolet absorbance
Experiment I-3 Protein quantification by BCA (Bichinchominic acid) method
Experiment I-4 Protein quantification by Lowry method
Experiment I-5 Enzyme Kinetics
ⅡGene analysis technology
Experiment II-1 Polymerase Chain Reaction (PCR)
Experiment II-2 Transformation and Confirmation of Genetic Traits
Experiment II-3: Purification of Plasmid DNA
Experiment II-4 Restriction Enzyme Treatment and Plasmid DNA Confirmation
Experiment II-5 RNA extraction and quantification
Experiment Ⅱ-6 RT-PCR (reverse transcription polymerase chain reaction)
Ⅲ Clinical Application Technology
Experiment III-1 Sugar Search Reaction
Experiment III-2 Cholesterol Quantification - Modified Zak-Henly Method
Experiment III-3 Quantification of Alanine Aminotransferase (ALT) in Serum
Experiment III-4: Fractionation and protein quantification of experimental animals (mouse, rat)
Experiment III-5 Cell culture and viability measurement (Cell culture and MTT assay)
Ⅳ Reference
GOODS SPECIFICS
- Date of publication: February 28, 2011
- Page count, weight, size: 187 pages | 188*254*20mm
- ISBN13: 9788957022573
- ISBN10: 8957022570
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